Proyectos

Materia investigada: Regulación transcripcional, microbiología, metilaciones, cobre.

Fondo de Innovación para la Competitividad - FIC2023- 6ta región.

La producción de semillas de sandía en Chile es una de las que genera mayores volúmenes (12,5%) y mejores precios (26 MM U$FOB) de exportación respecto del total de semillas exportadas. En los últimos 5 años su exportación ha aumentado considerablemente ocupando el segundo lugar en este mercado. Derivado del procesamiento de los frutos se genera un alto porcentaje de pulpa y cáscara; residuos no aprovechables como subproducto para otras industrias como cuarta gama y/o farmacéutica. El elevado contenido antioxidantes de la sandía representa una oportunidad para su extracción y uso en otras industrias. La solución innovadora permitirá reutilizar grandes volúmenes de la pulpa y cáscara, mitigando su disposición inadecuada y mejorando prácticas agrícolas y biotecnológicas. El objetivo de la propuesta es desarrollar un paquete tecnológico consistente en tres aplicaciones que permiten valorizar los residuos de cáscara y pulpa de sandía para la producción de nutracéuticos, bioenmienda de suelos provenientes de relaves mineros, y sustrato para el crecimiento de microorganismos. El proyecto busca generar innovaciones que promuevan la transformación de los residuos agrícolas, proyectando así nuevos negocios para los productores hortícolas en la industria de los alimentos dando valor agregado a los residuos derivados del procesamiento de semillas. Los resultados esperados de esta iniciativa son: Portafolio de al menos 2 ingredientes funcionales (Licopeno y Citrulina) desarrollados y caracterizados; validación técnica del ingrediente principal (Licopeno o citrulina) con actividad antioxidante; bioenmienda validada en un entorno operacional (campo), alcanzando el nivel de madurez tecnológica TRL7; análisis de mercado robusto que incluye un plan de escalamiento técnico de la bioenmienda; medio de cultivo validado en un entorno operacional (empresas), alcanzando el nivel de madurez tecnológica TRL7; y análisis de mercado robusto que incluye un plan de escalamiento técnico.

The field of remote sensing is experiencing an unprecedented acceleration. Besides the large public programs such as Sentinel (see e.g. https://sentinel.esa.int/web/sentinel/missions/sentinel-2), private actors are creating fleets of micro-satellites capable of monitoring of the earth with daily revisits. This abundant and cheap data is creating opportunities for developing novel applications for the monitoring of industrial and agricultural activity. The automatic exploitation of this data is bound to specific application domain knowledge, which requires a mastery of advanced techniques such as computer vision and machine learning, as well as expert knowledge in the field of agriculture. To do this, the team must master earth observation satellites, be able to define the adequate mathematical detection theories, and build on a deep knowledge of satellite image processing, while also including expert knowledge in agriculture. This project aims at uniting competences across the fields of computer vision and machine learning, remote sensing to address emerging applications in agronomy. This project will in addition foster the creation of reproducible research by adopting a reproducible research methodology thus contributing the resulting algorithms to the journal Image Processing On-Line (IPOL). The IPOL journal is an initiative to establish a clear and reproducible state-of-the-art in the domain of image processing and computer vision.

Los laboratorios de fabricación digital son espacios que cuentan con maquinaria y personal capacitado para facilitar el diseño y desarrollo de prototipos y para promover la innovación en productos, procesos y servicios. Se conciben como laboratorios que facilitan herramientas de fabricación avanzada y capacidades a la comunidad en general, pudiendo ser más enfocados a emprendedores, empresas e institutos de investigación. Una característica común es que sirven como plataforma para estimular el aprendizaje y la invención en la comunidad. Las máquinas y capacidades técnicas instaladas en estos laboratorios brindan la oportunidad de encontrar soluciones innovadoras a problemas comunes y ser incubadores de microemprendimientos que resuelvan problemas de forma innovadora y sustentable. El primer laboratorio de fabricación digital, junto con el concepto FabLab, aparece en el MIT (Massachussets Institute of Technology, Estados Unidos) en el año 2000. Actualmente, existe una red mundial de alrededor de 3000 FabLabs distribuidos en 5 continentes. En Chile se pueden encontrar 17 de estos laboratorios, la mayoría de ellos concentrados en la Región Metropolitana; 2 en la Región del Maule y ninguno en la Región de O’Higgins. La ausencia de un laboratorio regional está en concordancia con estadísticas del año 2016 que reportan apenas 118 m2 de espacios dedicados a innovación en la Región de O’Higgins frente a 27 936 m2 en la Región Metropolitana. En ese contexto, la Región de O’Higgins es la segunda región con menor superficie dedicada a innovación. La instalación de un laboratorio de fabricación digital en la Región de O’Higgins se identifica como una gran oportunidad para promover la innovación, brindando acceso a equipos y a capacitaciones sobre herramientas de fabricación avanzada a industrias y emprendedores regionales.

[proyecto ejecutado en 4 años, por haberse acogido a extensión de pandemia] Fluid impacts are present in a large variety of situations. For instance, the craters formed by rain--drops impacting the soil are relevant in agricultural applications. Also, wave--impact can damage coastal structures, and impact of sloshing--waves may produce over--turning of trucks or vessels that transport fluids. Therefore, the relevance (I would say the impact) of fluid impact goes from industry to environmental sciences. And also because of its beauty and scientific challenges, fluid impact is currently (and largely) studied by communities of physicist and applied mathematicians. As the field of fluid impact is vast, we focus in one particular problem: the bottle flip challenge, as (1) it provides more contoured problems to be tackled experimentally during the time limits of this proposal; (2) it could give insights about other relevant and applied problems; (3) as it already received press coverage worldwide, it is likely to have a large visibility of the results obtained. The bottle flip challenge is a game consisting in spinning a plastic bottle partially filled with water, in order to make it landing vertically after completing a single turn, or more. In recent years the challenge received huge attention in social media and some press coverage including Las Ultimas Noticias. In our opinion, such effervescence for a physical phenomenon relies in the counter-intuitiveness of the trick: as the bottle is turning, one expect it to continue turning until falling down, instead of the abrupt and stable stop in a vertical position that actually occurs. Some of the videos, magazine publication and the available physics article (Dekker et al., 2018), focus their attention in the conservation of angular momentum and the variations of momentum of inertia to explain the successful landing. Dekker et al. recognize that the physics of water sloshing is highly complex in itself and approached the problem by the side of classical mechanics. What we propose here, is indeed to take the challenge of fluid dynamics to carry conserved-quantities explanations to a greater depth. Our starting point is a high--speed camera recording of a successful throw and landing. There, one can observe at least two key fluid-dynamical events that contribute to the vertical stabilization of the bottle: (1) the impact of a water jet into the wall, strongly reducing the bottle-angular-momentum during the free traveling of the system, and (2) a violent redistribution of water taking place at landing, where water captures an important amount of the kinetic energy carried by the bottle. After describing these two key events, we can already summarize this proposal as a committed experimental study of both events, plus an effort to translate these ideas into a (engineering inspired) sloshing dynamics application. We propose first to study the landing stage asking the following question (Question 1): for a container partially filled with fluid, can fluid motion act as a shock-absorber for the impact? We propose to perform an experiment where the bottle is rotated on its vertical axis before it is released (also vertically). Then we will study the effect of fluid motion, by simply defining a restitution coefficient (valid at landing impact) and to see when the loss of bottle-energy is maximized. Bottle-energy loss implies fluid-energy absorption: a balance that will be experimentally checked. Maximal loss of bottle-energy indeed ensures greater bottle stability at landing. Then, we will focus on the effect of water--jet--impact asking Question 2: On which circumstances jet-impact may stabilize a freely rotating container? On one side, we will perform experiments of bottle throwing just as the challenge proposes (that is, throwing the bottle by hand). Also, we will construct a quasi-2D experiment, to perform computer-controlled rotations of the bottle in order to produce jet--impact on the bottle walls. In both cases, we will study angular momentum transfer and deviations from bottles without impact by filming with a high-speed camera and applying mass conservation models. In order to return to the general problem of fluid impact, our final question (3) is Can we take advantage of jet-impact to stabilize any moving container? Here we will apply the previous knowledge to the study a classical configuration exhibiting wave impact: a container subjected to horizontal excitation. After characterizing impact conditions in the solid container, we will study the consequences (in wall acceleration for instance) of having a freely moving wall.

La fabricación digital es un concepto que está revolucionando el modo en que se producen piezas y objetos. Hace referencia a procesos de manufactura en los que se usan máquinas controladas por una computadora para fabricar un objeto, previamente diseñado en algún software. La fabricación digital incluye tecnologías como impresión y escaneo 3D, corte láser y mecanizado CNC (control numérico computarizado); que junto al diseño CAD (diseño asistido por computadora) y programación permiten procesar archivos digitales para construir objetos tangibles. También se relaciona con el modelo educativo STEAM (ciencia, tecnología, ingeniería, arte y matemática) y con tecnologías que definen la próxima revolución industrial, la industria 4.0. La fabricación digital puede ser considerada un medio para desarrollar competencias como la creatividad, la colaboración y el trabajo en equipo, la proactividad y el emprendimiento. Numerosas experiencias internacionales y nacionales en fabricación digital han demostrado ser eficaces en fomentar competencias transversales en estudiantes, a diferencia del simple uso de dispositivos electrónicos (por ejemplo, smartphones). La eficacia de la fabricación digital radica en que, si bien también implica el uso de dispositivos electrónicos, pone el foco en conceptualizar, desarrollar y construir un producto físico. En consecuencia, esta nueva filosofía basada en el “aprender haciendo” aumenta la motivación, otorga autonomía y brinda competencias laborales fundamentales para el siglo XXI. La pandemia Covid-19 ha traído pérdidas irreparables, pero también grandes aprendizajes y desafíos tecnológicos. Se ha acelerado la transformación digital y se ha manifestado un gran potencial de desarrollo tecnológico local. Por otra parte, también se han visualizado brechas digitales y de género en la educación chilena. Desde el punto de vista del impacto en aprendizaje en contexto de pandemia, se ha determinado que la Región de O’Higgins podría ser una de las más perjudicadas por el cierre prolongado de los establecimientos educacionales (MINEDUC, 2020). Sumado a ello, es particularmente preocupante la diferencia, en detrimento de las niñas y las adolescentes, que ocurre con el desempeño en áreas STEAM, por lo crucial que estas resultan en las futuras oportunidades, nivel de ingresos y calidad de vida a la que podrán acceder (UNESCO, 2019). La Estrategia Regional de Innovación identifica la baja formación e incorporación de nuevas tecnologías 4.0 como una brecha que limita la puesta en marcha de proyectos innovadores y la asociatividad entre los actores regionales. Indicadores comunes para medir la efectividad de la innovación empresarial y emprendimiento tecnológico son instrumentos de propiedad industrial, como patentes, y surgimiento de empresas de base tecnológica. Las estadísticas de la Región de O’Higgins no son buenas. Según los últimos datos de INAPI, apenas el 1,33% de las patentes solicitadas en Chile provienen de la Región de O’Higgins. Por otro lado, no existen registros de emprendimientos regionales de base tecnológica. La incorporación de las tecnologías de fabricación digital en la formación de jóvenes makers puede fortalecer la educación STEAM, reducir la brecha digital y de género y potenciar los procesos de innovación empresarial y emprendimiento tecnológico en la Región de O’Higgins.

The phenomenon of a soft liquid drop eroding a hard stone surface over time, immortalized in the ancient proverb «dripping water wears away the stone,» presents a profound mechanical puzzle. While craters are common imprints of high-energy events, those formed by persistent, low-energy water dripping are exceptional. The impact energy of a single drop is far below the threshold required to plastically deform or fracture the material, yet erosion occurs. This project seeks to answer the fundamental question: How can water erode stone through dripping and create distinctive craters? While recent advancements in drop-impact dynamics have revealed that an impacting drop generates propagating fronts of intense, singular pressure and shear, these theories were developed for ideal, non-porous surfaces and are insufficient to explain the erosion. Our preliminary experimental work—which has successfully reproduced water-dripping craters on gypsum targets while failing to erode non-porous materials—points to a crucial, previously overlooked element: the porous nature of the target material. We discovered that erosion and the formation of a distinct surface microstructure of pores commence only after the substrate becomes fully saturated with water. This key finding suggests that the complex interaction between the impact-induced flow and the internal, liquid-filled pore structure is the primary driver of the erosion mechanism. This project will establish the first comprehensive experimental and theoretical framework for slow erosion in porous ma- terials by water dripping. We will investigate three potential and non-exclusive micro-mechanisms. The first is low-Reynolds accumulative erosion, where the impact pressure pumps liquid into the matrix, generating high shear stress along pore walls that slowly abrades material, a process whose rate is expected to be proportional to the wall shear stress. The second is the inter-pore propagation of pressure shocks; because the surface pressure front arrives at adjacent pore openings at slightly different times, large pressure gradients are generated within the saturated matrix, inducing mechanical fatigue and failure of inter-pore walls. The third is cavitation bursts, where the negative-pressure front trailing the initial impact shock— akin to an explosion’s blast wave—causes the formation and violent collapse of vapor bubbles. These collapses generate localized but highly destructive shock waves, a process potentially detectable via acoustic emissions. Our methodology integrates a novel, multi-scale experimental approach with robust theoretical modeling. An automated, custom-built setup, featuring a syringe pump for precise drop control and a photo-gate for impact counting and synchroniza- tion, tracks crater evolution over tens of thousands of reproducible impacts. An automated translation stage will move the sample between the impact zone and a characterization chamber for on-the-run 3D shape reconstruction via high-resolution laser profilometry and for mass measurement via an integrated load cell. This will be complemented by a suite of characteriza- tion techniques, including high-speed imaging to capture rare ejecta events, microscopic surface imaging, and advanced bulk imaging (X-ray Micro-Tomography, Scanning Electron Microscopy or Nuclear Magnetic Resonance) to visualize the internal 3D pore network and wear propagation. Experiments will mainly utilize natural materials like gypsum and selenite, as well as custom-fabricated synthetic porous samples (e.g., PDMS). These transparent, engineered samples will allow for direct flow visualization via Particle Image Velocimetry (PIV) to isolate and study specific mechanisms in a controlled environment. The theoretical work will couple established models for drop-impact pressure distributions with frameworks for flow in porous media, wall-shear erosion, and wave propagation. The goal is to develop predictive formulae for crater growth rates and their scaling with fluid and material properties, which can be validated against our extensive experimental data. By leveraging the research team’s expertise in drop-impact forces and tackling this 2,500-year-old question, this project will provide novel insights into fluid-solid interactions, wear on porous materials, and landscape evolution. It moves beyond prior studies, which used simplified substrates, to address the central role of porosity in this long-unsolved problem in continuum physics.

We investigate how rain-mediated mechanical processes influence the spread of pathogens under field conditions. While it is well established that water is a primary vector for bacterial movement between plants, few studies have examined the detailed hydrodynamic mechanisms involved, particularly in the context of leaf morphology, surface roughness, and microbial adhesion. This gap restricts our ability to develop predictive models and preventive strategies for managing rain-borne plant diseases. The project's general objective is to elucidate the coupling between raindrop impact dynamics and bacterial dispersal patterns on cherry leaves under realistic rainfall conditions. Specifically, it aims to (i) characterize the mechanical interaction between raindrops and cherry leaves using high-speed imaging and physical analysis to observe the dispersal patterns of Pseudomonas syringae pv. syringae (Pss). (ii) evaluate the spatial dispersal of Pss inoculated artificially onto cherry leaves at different concentrations under controlled temperature and rainfall conditions, and (iii) develop an integrative predictive model based on physical variables of rain-leaf interaction and experimentally measured environmental conditions to estimate the dispersal and severity of Pss attack. Methodologically, our study combines high-speed photography, controlled laboratory rain simulations, and microbiological assays. We will perform experiments in a custom-designed rainfall simulator allowing precise control of droplet size, velocity, and impact angle. Bacterial suspensions of Pseudomonas syringae—a pathogen commonly associated with cherry canker—will be applied to leaves under standardized conditions. The dynamics of droplet impact, splash formation, and secondary droplet ejection will be recorded at high temporal resolution to quantify mechanical energy transfer and spatial distribution of splashed particles. Parallel microbiological analyses will determine bacterial survival rates, concentration profiles, and the extent of leaf-to-leaf contamination. We will integrate these results into a predictive model linking rainfall characteristics to potential bacterial dispersal distances and infection probabilities. We aim to enhance our understanding of the biophysical coupling between rainfall and pathogen mobility, establish a set of empirical relationships for disease spread modeling, and provide practical recommendations for orchard management under varying climatic scenarios. By bridging the gap between plant pathology and fluid mechanics, this project will provide a mechanistic foundation for reducing rain-mediated bacterial diseases in high-value fruit crops, contributing to the sustainability and resilience of O'Higgins agriculture.

Water vapor is a key component of the hydrological cycle since it is directly involved in the production of precipitation (rain, snow, hail). The transport of water vapor from the tropics (20ºN-20ºS) is fundamental to produce precipitation in midlatitudes (30ºS-50ºS) were local amounts atmospheric moisture are lower than the water column precipitated during a typical storm. This is especially evident during extreme precipitation events, where precipitation accumulation can surpass 2 or 3 times the local atmospheric water vapor available. Extreme precipitation events (EPEs) are expected to increase due to the anthropogenic climate change, and therefore studies addressing the dynamics and forcing factors of these events are increasingly important. Current research examining the relationship between water vapor transport and precipitation in central-southern Chile have advanced in this direction. However, there is a lack of research aiming to understand water-vapor-precipitation process at the mesoscale, where changes in the order of hours associated to convection are important. Even more, despite many storms in central-southern Chile show convective characteristics (e.g. precipitation rates of 10 mm/h or larger), studies looking at the mesoscale processes has not been addressed so far, partially due to the lack of ground-based weather radars. As a result, this research proposal takes the challenge of studying the transport of water vapor and link it with precipitation processes (stratiform and convective) at the mesoscale level in central and southern Chile by using a suit of observations and numerical modeling. To determine the water vapor mechanisms involved in the precipitation processes, the study will employ an atmospheric moisture budget, which involves the balance between a storage term (precipitation in this case) and the linear interaction between local changes, advection, and convergence of water vapor following an air parcel. The budget will be computed using gridded data from a state-of-the-art atmospheric reanalysis (ERA5), numerical simulations with the Weather Research and Forecasting (WRF) model, and mathematical techniques such as finite differences and the trapezoidal integration rule. In addition, a relatively dense network of GPS deployed in central-southern Chile will provide direct estimates of local changes of the column water vapor, allowing us to perform a thorough validation of both ERA5 and WRF. Precipitation processes will be examined using several sources. The polar orbiting Global Precipitation Measurement (GPM) satellite mission provides global swaths of radar reflectivity using a dual-frequency radar (Ku and Ka bands) in a swath-width of 245 km with 5 km resolution at nadir, and vertical beams spaced at 250 m. Along with radar reflectivity, GPM provides estimates of precipitation rates and a classification of the precipitation type, facilitating the identification of precipitation processes. A vertically pointing precipitation radar (Micro Rain Radar, MRR) is currently installed at Universidad de Concepción and will provide time-height sections of radar reflectivity that will complement GPM observations. In addition, a second MRR is planned to be installed in central Chile to provide further meridional context of precipitation processes. Finally, a couple of optical disdrometers and meteorological stations will deliver surface estimates of precipitation at hourly (and higher) rates. In parallel, ERA5 will provide precipitation estimations and classification (stratiform, convective), while WRF will allow to examine precipitation in detail for selected case studies. At the end of this project, it will be clear what component(s) of the moisture budget are dominating precipitation during EPE storms, clarify the relative importance of stratiform and convective precipitation during EPEs, and elucidate if EPEs with strong convective precipitation are forced by atmospheric instabilities, advection of moisture being lifted by the complex terrain, or moisture convergence occurring over the ocean and moving inland. These results will provide the basis for future efforts looking to improve precipitation forecasting tools.