Proyectos

[vc_section el_class="container mx-auto align-items-center circle--pattern" css=".vc_custom_1648956589196{padding-top: 3rem !important;}"][vc_row el_class="pb-5"][vc_column][vc_wp_custommenu nav_menu="6"][uoh_breadcrumb_component automatic_breadcrumb="true"][uoh_title_component title_dropdown="big" title_decorator="true"]{{title}}[/uoh_title_component][vc_column_text css=""]Polycystic ovary syndrome (PCOS) is a prevalent and multifactorial endocrine disorder in women, which is characterized by reproductive and metabolic alterations that include hyperandrogenism, polycystic ovarian morphology, anovulation, insulin resistance, dyslipidemia and obesity with a high risk to develop type 2 diabetes and cardiovascular disease. However, the mechanisms that contribute to the development of metabolic abnormalities are not completely understood. Currently, DNA methylation, an epigenetic mechanism that regulates gene expression, has been proposed as an attractive possibility that might explain the heterogeneity of the syndrome linking the effect of environmental factors on susceptible genes to the presence of metabolic features. Data from women with PCOS and animal models of hyperandrogenism have found differential methylation patterns in several gene promoters related to glucose and lipid metabolism leading to a dysregulation of the expression of these genes and metabolic disorders. On the other hand, several environmental factors that may contribute with the establishment of these epigenetic modifications in women with PCOS remain unidentified. In this regard, it is widely accepted that different foods and nutrients have an impact on the regulation of epigenetic mechanisms, among them it has been proposed that elevated folic acid (FA) levels could induce an hypermethylation of the DNA which could reduce the expression of important regulatory genes including those asociated with metabolic function. Interestingly, women with PCOS show changes in folate and homocysteine levels suggesting an altered folate metabolism, which could be associated to changes in the methylation patterns of metabolic genes. In the present research project, we propose that androgen excess disrupts the folate cycle leading to folate excess in women with PCOS compared to control women. This alteration leads to hypermethylation of candidate genes involved in glucose and lipid metabolism (PPARG, FOXO1, GPAM and APOA2) which may impact the metabolic phenotype of women with PCOS. To test this hypothesis, 37 women with PCOS and 37 control women will be recruited to assess the effect of androgen excess in women with PCOS on folate metabolism circulating biomarkers such as folates, vit B12 and homocysteine (Hcy) (Specific aim 1). These results will be categorized according to quartiles of folate consumption according to the dietary pattern, using a validated food frequency questionnaire, and associated with circulating androgen levels. In addition, to evaluate whether androgen excess induces changes in folate cycle enzymes, gene expression analyses will be performed in peripheral blood mononuclear cells (PBMC) (Specific aim 2). To establish the causality of our hypothesis, we will evaluate the effect of androgen excess on the folate cycle using a cellular model. For this, gene and protein expression of the enzymes involved on the folate cycle (MAT, SAHH, MS, MTHFR, FOL1R y AHCY) will be analyzed in an hepatocyte cell line stimulated with testosterone (Specific aim 3). To check the specificity of androgen action we will use androgen blockers and aromatase inhibitors. Finally, to determine the effect on genes associated to glucose and lipid metabolism, DNA methylation in promoter regions and gene expression of candidate genes associated to glucose and lipid metabolism (PPARG, FOXO1, GPAM and APOA2) will be analyzed (Specific aim 4). We expect to find an increase in folate and homocysteine levels, and decreased levels of vitamin B12 in women with PCOS, asociated with a decreased expression of folate metabolism enzymes. On the other hand, in the in vitro model, we expect high levels of folic acid and a decrease in the expression of genes involved in folate metabolism in hepatocytes stimulated with androgens. This dysregulation in folate metabolism induced by androgen excess may result in hypermethylation of genes involved in lipid and glucose metabolism such as PPARG, FOXO1, GPAM and APOA2, leading to a lower gene expression which may impact the metabolic parameters of PCOS women. The results obtained from this proposal will help to identify new mechanisms involved in the pathophysiology of metabolic alterations in PCOS leading to the development of more effective strategies of prevention and therapy[/vc_column_text][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649209804184{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5"][vc_row el_class="container mx-auto align-items-center p-md-0 pt-5"][vc_column el_class="p-0"][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649210787516{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5 pb-md-5"][vc_row el_class="container mx-auto align-items-center"][vc_column][/vc_column][/vc_row][/vc_section]

[vc_section el_class="container mx-auto align-items-center circle--pattern" css=".vc_custom_1648956589196{padding-top: 3rem !important;}"][vc_row el_class="pb-5"][vc_column][vc_wp_custommenu nav_menu="6"][uoh_breadcrumb_component automatic_breadcrumb="true"][uoh_title_component title_dropdown="big" title_decorator="true"]{{title}}[/uoh_title_component][vc_column_text css=""]Polycystic ovary syndrome (PCOS) is a prevalent and multifactorial endocrine disorder in women, which is characterized by reproductive and metabolic alterations that include hyperandrogenism, polycystic ovarian morphology, anovulation, insulin resistance, dyslipidemia and obesity with a high risk to develop type 2 diabetes and cardiovascular disease. However, the mechanisms that contribute to the development of metabolic abnormalities are not completely understood. Currently, DNA methylation, an epigenetic mechanism that regulates gene expression, has been proposed as an attractive possibility that might explain the heterogeneity of the syndrome linking the effect of environmental factors on susceptible genes to the presence of metabolic features. Data from women with PCOS and animal models of hyperandrogenism have found differential methylation patterns in several gene promoters related to glucose and lipid metabolism leading to a dysregulation of the expression of these genes and metabolic disorders. On the other hand, several environmental factors that may contribute with the establishment of these epigenetic modifications in women with PCOS remain unidentified. In this regard, it is widely accepted that different foods and nutrients have an impact on the regulation of epigenetic mechanisms, among them it has been proposed that elevated folic acid (FA) levels could induce an hypermethylation of the DNA which could reduce the expression of important regulatory genes including those asociated with metabolic function. Interestingly, women with PCOS show changes in folate and homocysteine levels suggesting an altered folate metabolism, which could be associated to changes in the methylation patterns of metabolic genes. In the present research project, we propose that androgen excess disrupts the folate cycle leading to folate excess in women with PCOS compared to control women. This alteration leads to hypermethylation of candidate genes involved in glucose and lipid metabolism (PPARG, FOXO1, GPAM and APOA2) which may impact the metabolic phenotype of women with PCOS. To test this hypothesis, 37 women with PCOS and 37 control women will be recruited to assess the effect of androgen excess in women with PCOS on folate metabolism circulating biomarkers such as folates, vit B12 and homocysteine (Hcy) (Specific aim 1). These results will be categorized according to quartiles of folate consumption according to the dietary pattern, using a validated food frequency questionnaire, and associated with circulating androgen levels. In addition, to evaluate whether androgen excess induces changes in folate cycle enzymes, gene expression analyses will be performed in peripheral blood mononuclear cells (PBMC) (Specific aim 2). To establish the causality of our hypothesis, we will evaluate the effect of androgen excess on the folate cycle using a cellular model. For this, gene and protein expression of the enzymes involved on the folate cycle (MAT, SAHH, MS, MTHFR, FOL1R y AHCY) will be analyzed in an hepatocyte cell line stimulated with testosterone (Specific aim 3). To check the specificity of androgen action we will use androgen blockers and aromatase inhibitors. Finally, to determine the effect on genes associated to glucose and lipid metabolism, DNA methylation in promoter regions and gene expression of candidate genes associated to glucose and lipid metabolism (PPARG, FOXO1, GPAM and APOA2) will be analyzed (Specific aim 4). We expect to find an increase in folate and homocysteine levels, and decreased levels of vitamin B12 in women with PCOS, asociated with a decreased expression of folate metabolism enzymes. On the other hand, in the in vitro model, we expect high levels of folic acid and a decrease in the expression of genes involved in folate metabolism in hepatocytes stimulated with androgens. This dysregulation in folate metabolism induced by androgen excess may result in hypermethylation of genes involved in lipid and glucose metabolism such as PPARG, FOXO1, GPAM and APOA2, leading to a lower gene expression which may impact the metabolic parameters of PCOS women. The results obtained from this proposal will help to identify new mechanisms involved in the pathophysiology of metabolic alterations in PCOS leading to the development of more effective strategies of prevention and therapy[/vc_column_text][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649209804184{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5"][vc_row el_class="container mx-auto align-items-center p-md-0 pt-5"][vc_column el_class="p-0"][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649210787516{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5 pb-md-5"][vc_row el_class="container mx-auto align-items-center"][vc_column][/vc_column][/vc_row][/vc_section]

[vc_section el_class="container mx-auto align-items-center circle--pattern" css=".vc_custom_1648956589196{padding-top: 3rem !important;}"][vc_row el_class="pb-5"][vc_column][vc_wp_custommenu nav_menu="6"][uoh_breadcrumb_component automatic_breadcrumb="true"][uoh_title_component title_dropdown="big" title_decorator="true"]{{title}}[/uoh_title_component][vc_column_text css=""]The current study will quantify the level of employment in young adults with ASD and identify barriers to and facilitators of their labor inclusion. In addition, this study will determine if the level of stigma is associated with employment rates in young adults with ASD. The overarching goal is to contribute to improve the quality of life in adults with ASD in Chile by informing the development of public policies and interventions to increase the employment rate in this vulnerable population.[/vc_column_text][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649209804184{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5"][vc_row el_class="container mx-auto align-items-center p-md-0 pt-5"][vc_column el_class="p-0"][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649210787516{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5 pb-md-5"][vc_row el_class="container mx-auto align-items-center"][vc_column][/vc_column][/vc_row][/vc_section]

[vc_section el_class="container mx-auto align-items-center circle--pattern" css=".vc_custom_1648956589196{padding-top: 3rem !important;}"][vc_row el_class="pb-5"][vc_column][vc_wp_custommenu nav_menu="6"][uoh_breadcrumb_component automatic_breadcrumb="true"][uoh_title_component title_dropdown="big" title_decorator="true"]{{title}}[/uoh_title_component][vc_column_text css=""]The current study will quantify the level of employment in young adults with ASD and identify barriers to and facilitators of their labor inclusion. In addition, this study will determine if the level of stigma is associated with employment rates in young adults with ASD. The overarching goal is to contribute to improve the quality of life in adults with ASD in Chile by informing the development of public policies and interventions to increase the employment rate in this vulnerable population.[/vc_column_text][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649209804184{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5"][vc_row el_class="container mx-auto align-items-center p-md-0 pt-5"][vc_column el_class="p-0"][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649210787516{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5 pb-md-5"][vc_row el_class="container mx-auto align-items-center"][vc_column][/vc_column][/vc_row][/vc_section]

[vc_section el_class="container mx-auto align-items-center circle--pattern" css=".vc_custom_1648956589196{padding-top: 3rem !important;}"][vc_row el_class="pb-5"][vc_column][vc_wp_custommenu nav_menu="6"][uoh_breadcrumb_component automatic_breadcrumb="true"][uoh_title_component title_dropdown="big" title_decorator="true"]{{title}}[/uoh_title_component][vc_column_text css=""]Desarrollo de libro infantil sobre bienestar.[/vc_column_text][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649209804184{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5"][vc_row el_class="container mx-auto align-items-center p-md-0 pt-5"][vc_column el_class="p-0"][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649210787516{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5 pb-md-5"][vc_row el_class="container mx-auto align-items-center"][vc_column][/vc_column][/vc_row][/vc_section]

[vc_section el_class="container mx-auto align-items-center circle--pattern" css=".vc_custom_1648956589196{padding-top: 3rem !important;}"][vc_row el_class="pb-5"][vc_column][vc_wp_custommenu nav_menu="6"][uoh_breadcrumb_component automatic_breadcrumb="true"][uoh_title_component title_dropdown="big" title_decorator="true"]{{title}}[/uoh_title_component][vc_column_text css=""]Desarrollo de libro infantil sobre bienestar.[/vc_column_text][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649209804184{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5"][vc_row el_class="container mx-auto align-items-center p-md-0 pt-5"][vc_column el_class="p-0"][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649210787516{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5 pb-md-5"][vc_row el_class="container mx-auto align-items-center"][vc_column][/vc_column][/vc_row][/vc_section]

[vc_section el_class="container mx-auto align-items-center circle--pattern" css=".vc_custom_1648956589196{padding-top: 3rem !important;}"][vc_row el_class="pb-5"][vc_column][vc_wp_custommenu nav_menu="6"][uoh_breadcrumb_component automatic_breadcrumb="true"][uoh_title_component title_dropdown="big" title_decorator="true"]{{title}}[/uoh_title_component][vc_column_text css=""]Desarrollo de libro infantil sobre bienestar.[/vc_column_text][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649209804184{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5"][vc_row el_class="container mx-auto align-items-center p-md-0 pt-5"][vc_column el_class="p-0"][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649210787516{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5 pb-md-5"][vc_row el_class="container mx-auto align-items-center"][vc_column][/vc_column][/vc_row][/vc_section]

[vc_section el_class="container mx-auto align-items-center circle--pattern" css=".vc_custom_1648956589196{padding-top: 3rem !important;}"][vc_row el_class="pb-5"][vc_column][vc_wp_custommenu nav_menu="6"][uoh_breadcrumb_component automatic_breadcrumb="true"][uoh_title_component title_dropdown="big" title_decorator="true"]{{title}}[/uoh_title_component][vc_column_text css=""]Desarrollo de libro infantil sobre bienestar.[/vc_column_text][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649209804184{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5"][vc_row el_class="container mx-auto align-items-center p-md-0 pt-5"][vc_column el_class="p-0"][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649210787516{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5 pb-md-5"][vc_row el_class="container mx-auto align-items-center"][vc_column][/vc_column][/vc_row][/vc_section]

[vc_section el_class="container mx-auto align-items-center circle--pattern" css=".vc_custom_1648956589196{padding-top: 3rem !important;}"][vc_row el_class="pb-5"][vc_column][vc_wp_custommenu nav_menu="6"][uoh_breadcrumb_component automatic_breadcrumb="true"][uoh_title_component title_dropdown="big" title_decorator="true"]{{title}}[/uoh_title_component][vc_column_text css=""]Objetivo general: Analizar la relación entre las condiciones laborales y de empleo, con el bienestar psicosocial durante la Pandemia por COVID-19 en Chile con perspectiva de género. Objetivo(s) específico(s): - Caracterizar las modificaciones en las condiciones de empleo, trabajo y el bienestar psicosocial, según perspectiva de género, durante la pandemia por COVID-19, en Chile y en la Región de O'Higgins - Relacionar las condiciones de empleo y trabajo, y el bienestar psicosocial según perspectiva de[/vc_column_text][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649209804184{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5"][vc_row el_class="container mx-auto align-items-center p-md-0 pt-5"][vc_column el_class="p-0"][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649210787516{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5 pb-md-5"][vc_row el_class="container mx-auto align-items-center"][vc_column][/vc_column][/vc_row][/vc_section]

[vc_section el_class="container mx-auto align-items-center circle--pattern" css=".vc_custom_1648956589196{padding-top: 3rem !important;}"][vc_row el_class="pb-5"][vc_column][vc_wp_custommenu nav_menu="6"][uoh_breadcrumb_component automatic_breadcrumb="true"][uoh_title_component title_dropdown="big" title_decorator="true"]{{title}}[/uoh_title_component][vc_column_text css=""]Objetivo general: Analizar la relación entre las condiciones laborales y de empleo, con el bienestar psicosocial durante la Pandemia por COVID-19 en Chile con perspectiva de género. Objetivo(s) específico(s): - Caracterizar las modificaciones en las condiciones de empleo, trabajo y el bienestar psicosocial, según perspectiva de género, durante la pandemia por COVID-19, en Chile y en la Región de O'Higgins - Relacionar las condiciones de empleo y trabajo, y el bienestar psicosocial según perspectiva de[/vc_column_text][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649209804184{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5"][vc_row el_class="container mx-auto align-items-center p-md-0 pt-5"][vc_column el_class="p-0"][/vc_column][/vc_row][/vc_section][vc_section css=".vc_custom_1649210787516{background-color: #f6faff !important;}" el_class="p-md-0 pt-md-5 pb-md-5"][vc_row el_class="container mx-auto align-items-center"][vc_column][/vc_column][/vc_row][/vc_section]