Participé en este proyecto desde el 2010 hasta el 2013.

Objetivo general
Crear y transferir la tecnología necesaria que permita la producción y exportación a gran escala de cerezas de alta calidad en la zona Centro-Sur de Chile.

Objetivos específicos
Evaluar el potencial productivo y de calidad de variedades tardías de cerezas aptas de producir en la zona centro sur.; Desarrollar las de directrices la Producción Integrada de Cerezas en la zona CentroSur.; Transferir y difundir, comenzando desde una fase temprana, la tecnología que desarrollará el proyecto.; Proponer el diseno de un centro tecnológico y de difusión frutícola en la zona que basado en la tecnología propuesta por el proyecto, realice labores de investigación, desarrollo y transferencia tecnológica.

*El valor fue calculado en base a una media del convenio que se establece con las empresas químicas.

• CHEMIE Chile. Eficacia de DC-MAX SL para el control de Neofabraea sp., en manzanos orgánicos en la localidad de Bulnes, Chillán.
• ANASAC. Eficacia bactericida de Cobamin para el control de Xanthomonas arboricola pv. corylina en Corylus avellana, Región de La Araucanía. Chile.
• ANASAC. Eficacia bactericida de Cobamin para el control de hongos de poscosecha de avellana europea, Región de La Araucanía. Chile.
• CHEMIE Chile. Eficacia in vitro de DC-MAX SL para el control de Neofabraea sp.
• Basf Chile. Eficacia in vitro y in planta del producto comercial Comet EC contra Xanthomonas arboricola pv. corylina, Región de La Araucanía. Chile.
• Basf Chile. Eficacia in vitro del producto comercial Comet EC contra hongos de la semilla y estructuras reproductivas del avellano europeo, Región de La Araucanía. Chile.
• BASF Chile. Verificación de viabilidad de cepas de bacterias antagonistas contenidas en el producto Ecoterra SC. Chile.
• BioAmerica S.A. “Evaluación fitotóxica in vivo del bioestimulante BC-FE-12 en arándano”. Chile.
• Agrotechnology. “Eficacia del fungicida orgánico GWN9999 contra Botrytis cinerea en cultivares de arándano”. Chile
• Arysta Life Science Chile. “Eficacia in vivo de los fungicidas Tebuconazole y Caldera contra Neofusicoccum nonquaesitum y N. parvum en arándano”. Chile.
• Stoller. “Evaluación in vivo de Sett, Stimulate and X-cyte en arándano”. Chile.
• MIP Agro. “Eficacia in vivo de Ospobot contra Botrytis cinerea en arándano”. Chile.

Shoot blight, twig and branch cankers with grayish discoloration, roughened tissue, and dieback were observed in 5-year-old European hazelnut trees (Corylus avellana L.) cv. Barcelona at two commercial plantations in the Allipén locality, Region de La Araucanía (38° 59? 24.76? S, 72° 29? 35.58? W), Chile, during the 2011 to 2012 growing season. In addition, discoloration of the wood was observed in cross-sections. In order to isolate the causal agent, entire twigs and shoot fragments with cankers were sterilized in 0.5% sodium hypochlorite for 2 minutes, followed by two rinses with sterile distilled water. The tissues were then stored in a humid chamber. Sub-epidermal black pycnidia with sporulation were detected on the symptomatic tissue. Conidia were transferred to potato dextrose agar (PDA) (Difco, Lawrence, KS) and incubated at 25°C in the dark. The mycelia were black, creeping, and compact in appearance. The mature conidia were dark brown with a single septum, slightly constricted at the septum, and ovoid with a broadly rounded apex; some had a truncated base. Conidia had the following measurements: (20.0-) 23.1 ± 1.9 (-28.0) × (10-) 11.9 ± 1.2 (-15) mm with an average length/width ratio of 1.95 ± 0.17 (n = 50). These morphometric characteristics correspond to those of Diplodia coryli Fuckel (1870), teleomorph: Botryosphaeria sensu lato. The identity of the fungus was confirmed using internal transcribed spacer (ITS) rDNA sequencing completed at CABI, United Kingdom. The sequencing report indicated that the isolate (IMI-501235a) had 100% homology with a reference strain (CBS 242.51) in the CBS collection. The obtained sequence was deposited in GenBank (Accession No. JX163116). The anamorphs of Botryosphaeria have been divided into up to 18 genera (1), many of which are not clearly defined. Diplodia (3), including D. coryli (CBS 242.51) and D. juglandis (CBS 188.87), have been included within the genus Dothiorella (2), but the taxon names have not been formally changed. A pathogenicity test was conducted with one isolate (IMI-501235a) and four 1-year-old plants of hazelnut cultivar Barcelona. Plants were maintained in individual bags in greenhouse conditions (14/10 hours dark/light, 20°C; 70% RH). Prior to inoculation, plant tissues were externally disinfected with sodium hypochlorite (2%) and rinsed with sterile distilled water. Each plant was inoculated at fresh wound sites on two shoots and three twigs around each vegetative bud. The inoculum consisted of an agar plug with mycelia (5 mm) from the margin of an actively growing colony cultured on PDA media for 7 days. Each wound was covered with moistened cotton and sealed with Parafilm; a control plant was inoculated in the same way with agar only. After 3 months, fragments of necrotic and discolored vascular system tissues from inoculated shoots were removed and incubated on PDA. D. coryli was consistently recovered from these tissues, satisfying Koch’s postulates. The control plant showed no symptoms of the disease. D. coryli has been reported to cause symptoms of dieback (dead branches) in Italy and Spain. To our knowledge, this is the first report of D. coryli on C. avellana cv. Barcelona in Chile. European hazelnut is an emerging crop in Chile, grown mainly for export, and management strategies for this disease will need to be developed. References: (1) S. Denman et al. Stud. Mycol. 45:129, 2000. (2) A. J. L. Phillips et al. Persoonia 21:29, 2008. (3) A. J. L. Phillips et al. Mycologia 97:513, 2005.

• CBC (Europe) S.r.l. “Lotta biologica al cancro batterico dell’actinidia (P. syringae pv. actinidiae, Psa) con uso di batteri antagonisti”. Cesena (FC) – Italia.
• CBC (Europe) S.r.l. “Efficacy in vitro of the biofungicide Amylo-X against different bacterial pathogen strains (Xanthomonas spp., Pseudomonas spp., Erwinia spp.) in comparison with the efficacy of KSF40 and SB9501 biofungicides”. Cesena (FC) – Italia.
• Consorzio Kiwigold s.r.l. Consortile, Cesena (FC). “Analisi microbiologica di polline di Actinidia pre- e post- trattamento antibatterico”. Italia.
• Valagro, Spa. “Efficacy in vitro and in vivo of the product bioestimulante No. 3-2435 against Clavibacter michiganensis subsp. michiganensis, Xanthomonas vesicatoria e Pseudomonas syringae pv. actinidiae”. Italia.